【摘要】 目的　探讨干扰素 γ(IFN γ)对动脉损伤后平滑肌细胞 (SMC)增殖和迁移的作用及其作用机制。方法　建立兔髂动脉球囊损伤动物模型 ,培养损伤后动脉SMC ,实验分四组 :对照组、转化生长因子 β1(TGF β1)组、IFN γ组、TGF β1+IFN γ组。每组细胞加培养液或加入 10ng/mlTGF β1或 /和5 0 0U/mlIFN γ刺激 72h ,细胞计数和四甲基偶氮唑盐 (MTT)法测定各组SMC的增殖 ,同时测定SMC迁移。明胶酶谱分析SMC中MMP 2的活力。结果　与对照组比较 [细胞数 (2 .875± 0 .32 3)× 10 5个 /ml,迁移距离 (2 79.9± 8.12 9) μm],72h后TGF β1组细胞数 (4 .188± 0 .2 39)× 10 5个 /ml,(P <0 .0 1)和迁移距离 (36 5 .8± 9.6 86 ) μm ,(P <0 .0 1)增加 ,MTTA值检测细胞增殖抑制率为 - 19.4 % ;IFN γ组细胞数 (1.938± 0 .2 4 9× 10 5个 /ml,P <0 .0 1)和迁移距离 (2 34.4± 9.72 2 μm ,P <0 .0 1)减少 ,细胞增殖抑制率为 15 .8% ;TGF β1+IFN γ组细胞数 (3.12 5± 0 .2 5 4× 10 5个 /ml,P <0 .0 1)和迁移距离(32 3.1± 8.4 81) μm ,(P <0 .0 1)低于TGF β1组 ,细胞增殖抑制率为 - 9.1%。明胶酶谱分析显示各组细胞均可检测到基质金属蛋白酶 2 (matrixmetalloproteinase 2 ,MMP 2 6 6× 10 3 u) ,TGF β1组还可检更多还原

【Abstract】 Objective To investigate the role of interferon γon smooth muscle cells proliferation and migration after balloon injury. Methods Animal model of rabbit iliac artery balloon injury was set up, smooth muscle cells derived from injured artery were cultured. Smooth muscle cells were divided into four groups ( control, TGF β 1, IFN γ, TGF β 1 and IFN γ).Cells from each group were treated with medium or TGF β 1 (10 ng/ml) and/or IFN γ(500 u/ml) for 72 h separately. Smooth muscle cell proliferation was determined by cell count and MTT, migration of smooth muscle cells was also detected. Matrix metalloproteinase 2 was also detected by zymography. Results Our results showed that, compared with group control(2.875±0.323×10 5 cells/ml,279.9±8.129 μm), TGF β 1 increased cell count (4.188±0.239×10 5 cells/ml, P <0.01) and migration distance(365.8±9.686 μm , P <0.01), and cell proliferation inhibiting rate of MTT was -19.4% ; whereas IFN γ decreased cell count(1.938±0.249×10 5 cells/ml, P <0.01)and migration distance (234.4±9.722 μm, P <0.01), and cell proliferation inhibiting rate of MTT was 15.8%; TGF β 1 combined with IFN γincreased cell count (3.125±0.254×10 5 cells/ ml, P <0.01) and migration distance(323.1± 8.481μm , P <0.01), but the value was lower than that in cells treated by TGF β 1, proliferation inhibiting rate was -9.1%. Zymography showed that MMP 2 could be detected in all groups, and pro MMP 2 could also be detected in cells treated by TGF β 1. Compared with group control, the relative activity of MMP 2 in cells treated with TGF β 1, IFN γand TGF β 1 together with IFN γ were 143%, 95%, and 109% repectively. Conclusions IFN γ can inhibit smooth muscle cells proliferation and migration and reduce the stimulation of TGF β 1 on smooth muscle cells proliferation and migration. IFN γcan also inhibit MMP 2 activity and lesson the stimulation of TGF β 1 on MMP 2 activity.更多还原