【摘要】 目的　观察间皮细胞在培养状态下的形态学特征 ,测定其抗凝与纤溶作用的强度 ,为血管假体内腔铺被细胞的选择提供依据。　方法　取SD大鼠大网膜、主动脉、皮下结缔组织用作间皮、内皮及成纤维细胞的培养。倒置显微镜及扫描电镜下观察各自的形态特点。取 3类细胞第 3代融合后 4 8h的无血清培养液 ,放射免疫法测定 6 酮 PGF1α(前列环素的代谢产物 )的含量 ,发色底物法测定纤溶酶原激活物活性。　结果　在相同培养状态下 ,间皮细胞生长较快 ,表现出许多与内皮细胞相似的形态特点。间皮细胞培养液中 6 酮 PGF1α浓度显著高于成纤维细胞及内皮细胞培养液中的浓度 ,纤溶酶原激活物活性也高于成纤维细胞者 ,但与内皮细胞相比无显著性差异。结论　在相同培养状态下 ,间皮细胞具有与内皮细胞相似的结构与功能特点 ,可能为血管假体内腔铺被层的理想材料。更多还原

【Abstract】 Objective To observe the structure feature of cultured mesothelial cells, determine its anticoagulant and fibrinolytic activity, and provide a basis for selection of artificial vascular prostheses coating cells. Methods The greater omenta, aortae and subcutanenous connective tissue of SD rats have been taken for mesothelial cell, endothelial cell and fibroblast culture. The cultured mesothelial cells have been observed by light microscopy and electron microscopy. The 6 keto PGF 1α (the metabolite of prostacyclin)concentrations in medium were measured by rodioimmunoassay, Tissue plasminogen activator(t PA) activity was detected by chromogenic assay. Results The cultured mesothelial cells have many structure feature similar to endothelial cells. The average concentration of the 6 keto PGF 1α in mesothelial cell culture was higher than that in endothelial cells and fibroblasts culture, the t PA activity in mesothelial cells culture was also higher than that in fibroblasts, but there wasn’t a significant difference between mesothelial cells and endothelial cells.Conclusion Mesothelial cells have similar structure and functions comparing with endothelial cells. It may be an ideal coating lining for artificial vascular impants. [更多还原