【摘要】 目的　建立妊娠中晚期接触可卡因的小鼠动物模型 ,研究可卡因对后代脑发育是否具有持续影响。方法　称量实验组与对照组仔鼠在P10的体重与脑重 ;分别采用甲苯胺蓝染色和免疫组织化学方法 ,观察各组仔鼠大脑皮质神经元和胼胝体与海马神经胶质细胞的发育情况。　结果　可卡因处理组的仔鼠P10体重与脑重比对照组明显降低 ;神经元极性紊乱、各皮层结构不清 ,神经元发育不良 ;GFAP免疫组织化学染色显示胼胝体区与海马区星形胶质细胞含量降低。　结论　妊娠中晚期暴露于可卡因对后代脑的发育具有长期影响 ,引起形态学的持续改变 ,这种影响可能在后代表现出的神经行为异常发病机制中扮演着重要角色。更多还原

【Abstract】 Objective To study the effects of prenatal cocaine exposure on the development of offspring’s brains by building a murine model. Methods We weighted the body weight and brain weight of offspring on P10 from COC and SAL groups and observed the development of neuron and astrocyte in cerebral cortex by toluidine blue staining and immunohistochemistry. Results The brain weight and body weight from COC were both reduced on P10 compared with SAL group.We discovered prenatal cocaine exposure induced polarity disorder and dysplasia of neuron in cerebral cortex;the number of the astrocytes in corpus callosum and hippocampus regions decreased.Conclusion\ Pregnatal cocaine exposure can result in abnormal development of cerebral cortex of offsprings which may play an important role on cocaine induced abnormal behavior.\;[更多还原