【摘要】 以银杏幼叶为外植体 ,对银杏进行愈伤组织诱导和继代培养。通过正交试验设计 ,研究了不同浓度的 3种激素配合使用对银杏愈伤组织诱导的效果 ,筛选出诱导银杏愈伤组织的最佳培养基 :MS+ 3 mg/LNAA+ 3 mg/L6-BA。研究了在银杏愈伤组织继代过程中 ,不同浓度的 4种抗氧化剂对褐变抑制的效果 ,筛选出最佳抗氧化剂及其浓度 :10 mg/L Vc。4种抗氧化剂对抑制褐变的效果如下 :Vc>PVP>柠檬酸 >Ac,其中 ,PVP的最佳浓度为 1g/L。继代过程中 ,光照条件对愈伤组织褐变程度有一定的影响。更多还原

【Abstract】 In the experiment, we induced callus with the tender leaves of ginkgo as explants and did subsculture. Through orthogonal test, the results showed that the best medium was MS+3mg/LNAA+3mg/L6-BA. Four antioxidants were used to prevent browning in the subculture. The results showed that effects of different antioxidants on browning are different, and from high to low is Vc, PVP, citric acid, Ac. The optimal concentration of VC is 10mg/L, and PVP is 1g/L. Culture conditions have some effects of browning. Condition of being in darkness is better.更多还原