【摘要】 目的 :克隆人载脂蛋白A1 (apoA1 )基因。　方法 :选择人胎肝组织 ,提取总RNA ,以此为模板 ,利用RT PCR法获取apoA1成熟肽基因片段 ,琼脂糖电泳分离纯化所得目的基因并克隆至T Vector ;ABI 377自动序列分析仪分析所得基因序列。　结果 :PCR产物经琼脂糖电泳 ,目的基因片段与预期大小相符 ;序列分析仪分析所得基因序列 739bps,与国外报道的完全相同。　 结论 :从人胎肝组织成功克隆apoA1基因更多还原

【Abstract】 Objectives:Identification, clone and sequence of the chinese mature apoA1 peptide gene.Methods:Total RNA was prepared from Chinese fetal liver tissue, cDNA fragment encoding human apoA1 was amplified by RT PCR using specific primers, and cloned into pGEM T vector. Inserted apoA1 gene was sequenced by ABI 377 DNA Sequencer. Results:Analysis indicates that the DNA fragment is 739 base pairs in length and has 100% nucleotide homology with that reported previously. Conclusions:Human apoA1 gene was successfully cloned from fetal liver tissue.更多还原