【摘要】 介绍了1种Ri质粒的快速提取方法,提取的质粒可用于目的基因的扩增,应用rolC基因的特异性引物,扩增出了正确序列的rolC基因0 87kb片段,并利用pGEM-T载体对此片段进行了克隆。更多还原

【Abstract】 A procedure for rapid extraction of Ri plasmid was developed and a 087 kb fragment of rolC gene was amplified by PCR via primer of rolC gene, and the fragment was cloned into vector pGEM-T The result of sequencing showed that the fragment was exactly rolC gene of TDNA of Ri plasmid更多还原