【摘要】 目的　探讨有机磷 (OP)中毒家兔脑干损伤的病理机制。方法 :2 4只青紫蓝家兔分 4组 :敌百虫 (1 0LD ,1 1 1 2 0mg/Kg)组 ,久效磷 (1 0LD ,2 2 2 4mg/Kg)组 ,甲基对硫磷 (1 0LD ,74 1 0mg/kg)组和正常对照组 ,每组 6只。中毒 6、 2 4h各取脑干组织用于组织病理学观察及制备组织匀浆。用酶抑制法测脑干组织游离有机磷毒质 (FOP)含量 ,用放射免疫法测组织中肿瘤坏死因子 α (TNF -α)、白细胞介素 1 β (IL 1 β)及血栓素B2 (TXB2 )含量。结果　中毒后 6、 2 4h脑干组织出现严重的病理损伤 ,可见脑干组织水肿、出血 ,神经细胞变性坏死 ,形成鬼影细胞 ,并见神经纤维脱髓鞘改变。同时 3种OP在脑干组织中可检出不同浓度的FOP。中毒后 6h ,脑干TXB2 含量高于正常 (t =3 984 3,P <0 0 1 ) ,2 4h与 6h比较差异有显著性 (t =2 5 4 32 ,P <0 0 5 ) ;TNF α、IL 1 β在各时相与正常比较差异无显著性 (P >0 0 5 )。结论　重度有机磷中毒可致动物脑干组织出现严重的病理损伤 ,除中毒酶机制以外 ,FOP和TXA2 在脑干损伤中具有重要作用更多还原

【Abstract】 Objective To explore the molecular and pathological mechanism of injury of brain stem injury in rabbits poisoned with organophosphates.Methods 24 livid blue rabbits were divided into 4 groups:trichlorfon(10LD,1 112 0 mg/kg),monocrotophos (10LD,22 24 mg/kg)and methy1 parathion(10LD,74 10 mg/kg)groups and control group.Every group consists of 6 rabbits.The organophosphates were administered by subcutaneous route.The specimens of brain stem were collected for pathological examination and preparation of tissue homogenate at 6 and 24 h after poisoning.The levels of free organophosphorus poisons(FOP) in brain stem were determined with enzyme inhibition method.The concentrations of tumor necrosis factor alpha(TNF α),interleukin 1 beta(IL 1β),Thromboxane B 2(TXB 2) in the brain stem were measured by radio immunoassay technique.Results Severe pathological changes of brain stem were observed at 6 and 24 h after poisoning,such as tissue edema,and hemorrhage,with degeneration and necrosis cells and neurofibrillar demyelination.There were different concentrations of FOP of trichlorfon,monocrotophos and methy1 parathion in the brain stem.TXB 2 level in the brain stem at 6 h after poisoning was significantly higher than that in control group ( t = 3 9843, P <0 01),and a significant difference was also found between 6 and 24 h after poisoning( t =2 5432, P <0 05).TNF α and IL 1β concentrations were not higher than those in control group( P >0 05).Conclusion Severe organophosphorus poisoning can result in severe pathological injury of the brain stem in animals.FOP and TXA 2 play important roles in the injury of the brain stem.更多还原