【摘要】 目的　分析输卵管妊娠患者巨细胞病毒感染及基因型的关系。方法　ELISA法检测 1 2 5例输卵管妊娠患者和 4 2例行附件切除术的无异位妊娠史的卵巢囊肿患者 (对照组 )血清中抗巨细胞病毒IgM ,生殖道组织以巢式聚合酶链反应 (nested PCR)测定巨细胞病毒糖蛋白gBn基因并测序分析基因型。结果　抗巨细胞病毒IgM阳性 1 8例 (1 4 % ) ,对照组阳性 1例 (2 % )。巨细胞病毒gBn基因阳性 2 5例 (2 0 % ) ,对照组 2例 ,巨细胞病毒检出率明显高于正常对照组 ,两组间差异有显著性意义。gBn基因分型为gBn 1型 3/1 6例 (1 9% ) ,2型 1 /1 6例 (6 % ) ,3型 1 1 /1 6例 (6 9% ) ,1 /3混合型 1 /1 6例 (6 % ) ,未见 4型 ,gBn基因型分布以gBn3型为主 (χ2 =1 2 2 86 ,P =0 0 0 6 )。结论　输卵管妊娠患者存在巨细胞病毒感染 ,gBn基因型分布以gBn3型为主 ,输卵管妊娠患者巨细胞病毒感染可能与其发生有关更多还原

【Abstract】 Objective To investigate the relationship between genital cytomegalovirus infection and the gBn genotype of human cytomegalovirus on the incidence of tubal pregnancy.Methods Serum cytomegalovirus IgM was determined by ELISA in 125 women with tubal pregnancy and 42 cases (served as control group)with ovarian cyst conducted by adnexectomy.Cytomegalovirus gB N terminus (gBn,54 485 nt) gene in reproductive tissues were amplified by nest PCR and PCR respectively. Positive samples were directly sequence analysis to identify the gB genotype.Results Positive rate of cytomegalovirus IgM and gB gene were 14% (18/125) and 20% (25/125), respectively,in women with tubal pregnancy, and 2% (1/42) and 5% (2/42),respectively,in control group. Positive rate of cytomegalovirus IgM and gB gene were found to be significantly higher in women with tubal pregnancy than those in control group .Sequence analysis of 16 positive gBn fragments showed that 3 cases (19%) were gBn1 genotypes, 2 cases (6%) gBn2 genotypes , 11 cases (69%) gBn3 genotypes, 1 case (6%) was co infected with gBn1 and gBn3 genotype, gBn4 genotype was not detected. gBn3 genotype was dominant .Conclusion The infective rate of cytomegalovirus in women with tubal pregnancy is significantly higher than that in control group. Human cytomegalovirus gBn3 genotype is dominant. It is suggested that genital cytomegalovirus infection may be associated with the incidence of tubal pregnancy. [更多还原