【摘要】 报道一种单分子荧光涨落检测系统的实验装置的设计。该系统主要由激光共焦系统、微区激发系统、光电转换及数据采集和分析系统组成。本文另对共焦系统和数据采集系统的原理作了简要的说明。由于采用了共焦系统 ,这样就可以从一个视场深度比较窄的区域内采集生物信息 ,而不需要象普通荧光采集系统那样大范围采集 ,焦点外的荧光可以直接消除 ,结果对比度、清晰度、灵敏度都增加了。加上先进的数据采集及处理系统 ,因而可实现在分子水平上对荧光信号进行检测 ,这些工作也为在单分子水平上探索疾病机理和揭示生命现象的本质提供了一个有力的手段更多还原

【Abstract】 It is described the work principle and development of the single molecules fluorescence fluctuation processing system.the system in composed of a confocal system,a data acquisition processing system.The system offers several advantage over conventional fluorescence processing system,The shallow depth of field allows information to be collectioned from a well-defined optical section rather than from most of the specimen as in the conventional fluorescence processing system,Out-of-focus fluorescence in virtually eliminated,which results in an increase in contrast clarity and detection sensitivity.This technique of applying the system will be a great significance for diagnositics and evolutionary biotechnology in single molecules level.更多还原