【摘要】 观察肝细胞生长因子 (HGF)及血管内皮细胞生长因子 (VEGF)对牛冠状动脉血管内皮细胞 (BCAEC)和平滑肌细胞(BCASMC)增殖的影响。分离和培养BCAEC、BCASMC ,VEGF组和HGF组细胞培养液分别含VEGF 5 0ng/ml和HGF 5 0ng/ml,采用四甲基偶氮唑蓝法观察细胞的增殖。结果显示 ,对照组、HGF组和VEGF组BCAEC的OD值分别为 0 2 3± 0 0 2、0 5 8± 0 10和 0 4 2± 0 12 ;BCASMC的OD值分别为 0 31± 0 0 8、0 4 5± 0 0 9和 0 4 0± 0 11;HGF对BCAEC、BCASMC的增殖率分别为 15 2 2 %± 33 8%、45 2 %±2 5 3%,VEGF对BCAEC、BCASMC的增殖率分别为 82 6 %± 18 7%、2 9 0 %± 2 0 4 %。提示HGF对BCAEC、BCASMC的增殖均有促进作用 ;VEGF能促进BCAEC的增殖 ,而对BCASMC的增殖作用不明显。HGF对BCAEC的增殖作用强于对BCASMC的作用 ;HGF对BCAEC的增殖作用强于VEGF。更多还原

【Abstract】 The effects of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) on proliferation of bovine coronary artery endothelial cells (BCAEC) and smooth muscle cells (BCASMC) were studied in vitro. BCAEC and BCASMC were isolated and cultured and divided into control group, VEGF (50ng/ml) group and HGF (50ng/ml) group. Cells proliferation was measured using MTT method. The results showed that the OD values of control, VEGF, and HGF group in BCAEC cultures were 0.23±0.02, 0.58±0.10, and 0.42±0.12, respectively, and those in BCASMC were 0.31±0.08, 0.45±0.09, and 0.40±0.11, respectively. The proliferation ratios of BCAEC and BCASMC induced by HGF were 152.2%±33.8% and 45.2%±25.3%, respectively, and that by VEGF were 82.6%±18.7% and 29.0%±20.4%, respectively. The results suggested that HGF could promote proliferation and migration of BCAEC and BCASMC, while VEGF could promote proliferation of BCAEC but not BCASMC. The effect of HGF on BCAEC was stronger than that on BCASMC, and the induction strength of HGF was higher than VEGF.更多还原