【摘要】 应用MTT法体外观察不同浓度茶多酚对PG细胞的杀伤作用 ,激光共聚焦显微镜和流式细胞仪检测用药后PG细胞内Ca2 +浓度、细胞间隙连接通讯(GJIC)、Cx4 3表达和细胞增殖周期分布的变化。结果显示 ,4个浓度的茶多酚对PG细胞均有杀伤作用 ,呈剂量依赖关系。细胞增殖受到明显抑制 ,使细胞阻滞于G0 /G1期 ,不能进入S期及G2 /M期 ,细胞增殖指数明显下降。与对照组相比 ,随着茶多酚浓度的增加 ,细胞内Ca2 + 浓度、GJIC和Cx4 3表达水平逐渐上升。提示茶多酚对PG细胞具有生长抑制作用 ,其机制可能与上调细胞间隙连接通讯功能有关更多还原

【Abstract】 The anti proliferation effects of four different concentrations of tea polyphenols on PG cell lines were determined with MTT assay. Laser scanning confocal mircroscopy and flow cytometry techniques were used to determine the changes in intracellular calcium concentration, GJIC, Cx43 expression, and cell cycle distribution after treatment with tea polyphenols. The results showed that four different concentrations dose dependently inhibited the proliferation of PG cell lines. It caused a decline in the proportion of cells in S and G2/M phase, blocked the cell cycle progression in G0/G1 phase, and reduced the proliferation index of PG cell lines. Compared with control group, intracellular calcium concentration, GJIC and Cx43 expression were gradually increased ascended with an increase in tea polyphenols concentration. The results suggested that tea polyphenol could inhibit growth of PG cell lines. The mechanism of anti tumor is associated with an up regulation of GJIC of PG cell line.更多还原