【摘要】 探讨γδT细胞在支气管哮喘外周血和支气管肺泡灌洗液 (BALF)中的分布和凋亡状态。应用鸡卵清蛋白(OVA)致敏和刺激Wistar大鼠 (每组 10只 ) ,制作致敏大鼠哮喘模型 ,收集外周血单个核细胞 (PBMC)和BALF ,采用流式细胞术检测γδTCR+ T细胞百分率和CD2 8 γδTCR平均荧光密度比 ,并用免疫荧光法结合HE染色以及免疫组化法检测γδT细胞占淋巴细胞的百分率 ,用TENUL法检测淋巴细胞凋亡。哮喘组PBMC中γδT细胞占总T细胞或总淋巴细胞比例明显低于正常组 (P <0 0 5 ) ,CD2 8 γδTCR平均荧光密度比则无明显差别 ;BALF中γδT细胞占总T细胞或总淋巴细胞比例明显高于正常组 (P <0 0 1) ,CD2 8 γδTCR平均荧光密度比也有显著增加 (P <0 0 1) ;哮喘组PBMC和BALF中淋巴细胞凋亡指数明显低于正常对照组 (P <0 0 1)。提示γδT细胞亚群参与了哮喘的发病过程。更多还原

【Abstract】 To explore the distribution and the active state of γδT cells in peripheral blood and bronchoalveolar lavage fluid(BALF) of asthma patients. Study the effect of γδT cell subtypes in mechanisms of asthma. Wistar rats (n=10,for each group) were sensitized and challenged with ovalbumin. Collect the PBMC and BALF. The percentage of γδT cells in total T cells and the ratio of the mean fluorescence density of CD28/γδTCR were detected by flow cytometry. The percentage of γδT cells in total lymphocytes was detected by immunofluorescence technique and immunohistochemistry. The apoptosis of lymphocytes was detected by TUNEL.In asthmatic group, the percentage of γδT cells among total T cells or total lymphocytes showed significant decrease compared to the control group ( P <0.05), but the mean fluorescence density of CD28/γδTCR did not showed any significant difference compared to the control group in PBMC. The percentage of γδT cells among total T cells or total lymphocytes also increased significantly ( P <0.01) compared to the control group, and the mean fluorescence density of CD28/γδTCR increased significantly ( P <0.01) compared to the control group in BALF. The apoptosis index(AI) of lymphocytes in PBMC and BALF of asthmatic group were both significantly decreased ( P <0.01) compared to the control group. The results suggested that the γδT cell subtypes was involved in the process of asthma.更多还原