节点文献
Structure, expression pattern and chromosomal localization of the rice Osgrp-2 gene
【Abstract】 Glycine-rich proteins (GRPs) belong to a kind of important structural proteins of plant cell walls. The expression of GRP genes is regulated spatially and developmentally as well as by various environmental stresses, thus providing a good model for the study of plant gene expres-sion. We obtained the genomic sequence of a new GRP gene (Osgrp-2) from a rice genomic li-brary. The transcription start site of Osgrp-2 was determined by 5’-rapid amplification of cDNA ends (RACE) and a 2.4-kb promoter sequence was thus delimited. The spatial and developmental expression pattern as well as the wound-inducible character of Osgrp-2 in rice plants was ana-lyzed in detail. Furthermore, the gene was mapped onto rice chromosome 10 by analysis of re-striction fragment length polymorphism (RFLP).
【Key words】 Oryza sativa; GRP; gene structure; expression pattern; chromosomal localization.;
- 【文献出处】 Science in China(Series C:Life Sciences) ,中国科学(C辑:生命科学)(英文版) , 编辑部邮箱 ,2003年06期
- 【分类号】S511
- 【被引频次】1
- 【下载频次】55