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Structure, expression pattern and chromosomal localization of the rice Osgrp-2 gene

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【作者】 刘宗旨王建龙王群黄勋徐卫辉朱立煌何平方荣祥

【Author】 LIU Zongzhi 1*, WANG Jianlong 1*, WANG Qun 1, HUANG Xun1, XU Weihui 1, ZHU Lihuang 2, HE Ping 2 & FANG Rongxiang 1 1. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China; 2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

【机构】 Institute of MicrobiologyChinese Academy of SciencesBeijing 100080ChinaInstitute of Genetics and Developmental BiologyBeijing 100101China

【Abstract】 Glycine-rich proteins (GRPs) belong to a kind of important structural proteins of plant cell walls. The expression of GRP genes is regulated spatially and developmentally as well as by various environmental stresses, thus providing a good model for the study of plant gene expres-sion. We obtained the genomic sequence of a new GRP gene (Osgrp-2) from a rice genomic li-brary. The transcription start site of Osgrp-2 was determined by 5’-rapid amplification of cDNA ends (RACE) and a 2.4-kb promoter sequence was thus delimited. The spatial and developmental expression pattern as well as the wound-inducible character of Osgrp-2 in rice plants was ana-lyzed in detail. Furthermore, the gene was mapped onto rice chromosome 10 by analysis of re-striction fragment length polymorphism (RFLP).

  • 【文献出处】 Science in China(Series C:Life Sciences) ,中国科学(C辑:生命科学)(英文版) , 编辑部邮箱 ,2003年06期
  • 【分类号】S511
  • 【被引频次】1
  • 【下载频次】55
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