【摘要】 用电化学方法和荧光光谱法,UV光谱法对Ce( )-ALC络合物与鱼精子DNA的相互作用进行了研究,发现在在pH为4.3的六次甲基四胺((CH2)6N4)缓冲溶液中,Ce( )-ALC络合物与鱼精子DNA(hsDNA)以嵌入方式生成一种电化学非活性络合物Ce( )-ALC-DNA,导致Ce( )-ALC的峰电流降低,峰电流在一定范围内与DNA的浓度成线性关系,线性范围为1～10μg/mL,相关系数为0.997,检测限为0.1μg/mL,因此该法可以用于DNA的测定.更多还原

【Abstract】 In this paper, the interaction of Ce(Ⅲ)ALC and herring sperm DNA(hsDNA) is studied by electrochemical, fluorimetric and UVspectrophotometric methods. Ce(Ⅲ)ALC complex can react with hsDNA to produce an electrochemically nonactive supermolecular complex Ce(Ⅲ)ALCDNA in the mode of intercalation in the buffer solution of (CH2)6N4(pH=4.3), which results in the decrease of the peak current of Ce(Ⅲ)ALC. The decrease of the peak current is proportional to DNA concentration from 1 to 10 μg/mL, the limit of detection is 0.1 μg/mL, and the correlation coefficient is 0.997, this method can be applied to determine DNA concentration.更多还原