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哈维氏弧菌的血清型与菌苗抗原性的关系

Relationship between the Serotype of Vibrio harveyi and the Antigenicity of the Bacterins

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【作者】 毛芝娟楼丹吴雄飞楠田理一陈昌福

【Author】 Mao Zhijuan Lou Dan Wu Xiongfei Riichi Kusuda Chen Changfu(Ningbo Fishery Research Institute, Ningbo 315012, China;Faculty of Engineering , Fukuyama University , Fukuyama, 729-02, Japan;Huazhong Agricultural University, Fishery College, Wuhan 430070, China;State Key Laboratory of Microbiology , Huazhong Agricultural University, Wuhan 430070, China )

【机构】 宁波市水产研究所,宁波市水产研究所,宁波市水产研究所,日本国福山大学工学部海洋生物工学科,华中农业大学水产学院 宁波 315012,宁波 315012,宁波 315012,福山 729-02,武汉 430070华中农业大学农业微生物国家重点实验室,武汉 430070

【摘要】 采用不同血清型哈维氏弧菌Vibrio harveyi 624、809和106等3个菌株,制备成福尔马林灭活菌苗,注射接种大黄鱼4周后,通过血清中凝集、交叉凝集抗体效价测定以及活菌攻毒试验,证明了3个菌株制备的菌苗抗原性存在差异。

【Abstract】 Formalin killed cells of Vibrio harveyi were prepared with three strains:Y624, Y809 and Y106, which represented different serotypes. The difference of the antigenicity among the bacterins prepared with these three strains was investigated by monitoring of agglutinating antibody titer. High agglutinating antibody titer was detecled againsl immunized antigen, but low cross-agglutinating anti-body liter was found. Challenge test with live pathogenic bacteria resulted in high survival percent a-gainst the corresponding one but low percent to the other two strains. It was suggested that the antigenicity of the bacterins prepared with the three strains was differenl.

【关键词】 哈维氏弧菌血清型抗原性
【Key words】 Vibrio harveyiserotypesantigenicity
【基金】 宁波市青年科技基金项目(991104);国家自然科学基金项目(30271032)资助
  • 【文献出处】 华中农业大学学报 ,Journal of Huazhong Agricultural , 编辑部邮箱 ,2003年06期
  • 【分类号】S948
  • 【被引频次】11
  • 【下载频次】213
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