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三肽囊素抗独特型抗体可变区基因库的建立
Construction of Gene Library of the Bursin Anti-idiotype Antibody Variable Region
【摘要】 利用抗bursin单克隆抗体免疫SPF来航鸡 ,获得抗独特型抗体的产生细胞 ,从中提取总RNA ,经反转录合成cDNA第一链。以cDNA第一链为模板 ,根据鸡抗体重、轻链基因设计引物 ,进行PCR扩增。在体外成功地扩增出该抗体的可变区重链和轻链基因。
【Abstract】 Spleenil cells generated from anti-idiotype antibody were obtained from SPF chicken immunized with McAb against bursin. Total RNA was isolated from these cells and cDNA was synthesized by reverse transcription. Two sets of oligonucleotide primer were designed according to constant region gene sequences of chicken immunoglobulin variable region. The repertoire of V Hand V Lgene fragments was amplified with cDNAs’ templates successfully.
【关键词】 三肽囊素;
抗独特型抗体;
可变区重链和轻链基因;
【Key words】 bursin; anti-idiotype antibody; variable region gene of heavy chain andlight chain;
【Key words】 bursin; anti-idiotype antibody; variable region gene of heavy chain andlight chain;
【基金】 江西省自然科学基金资助项目 ( 0 13 0 0 2 3 );江西省科技厅农业科技攻关项目资助
- 【文献出处】 华中农业大学学报 ,Journal of Huazhong Agricultural , 编辑部邮箱 ,2003年04期
- 【分类号】S852.4
- 【被引频次】1
- 【下载频次】64