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南沙群岛微型与超微型真核藻类遗传多样性的初步研究
PRIMARY STUDY OF GENETIC DIVERSITY OF EUKARY-OTIC MICRO AND PICOPLANKTON IN NANSHA ISLANDS
【摘要】 用分子生物学方法建立了南沙海域5号采样点附近海域的微型、超微型真核藻类185rDNA文库,采用RFLP和基因测序的手段对其遗传多样性进行了初步探讨。研究表明南沙海域的微型、超微型藻类的遗传多样性十分丰富,而且尚有大量未获培养的、分类位置未知的物种有待研究。
【Abstract】 Genetic libraries of 18S rRNA genes of eukaryotic micro and picoplankton from Nansha Islands were constructed by the molecular biological method in the paper. The genetic diversity of the libraries was screened by restriction fragment length polymorphism analysis and genetic sequencing. The results revealed the remarkable genetic diversity of eukaryotic micro and picoplankton from Nansha Islands, and there are an abundance of uncultured, unknown species in this oceanic region.
【关键词】 南沙;
微型、超微型藻类;
18S rDNA;
遗传多样性;
【Key words】 Nansha Islands; Micro and picoplankton; 18S rRNA genes; Genetic diversity;
【Key words】 Nansha Islands; Micro and picoplankton; 18S rRNA genes; Genetic diversity;
【基金】 国家自然科学基金项目39970063号、40076031号;广东省自然科学基金011208号、01213号
- 【文献出处】 海洋科学 ,Marine Sciences , 编辑部邮箱 ,2003年07期
- 【分类号】Q943
- 【被引频次】21
- 【下载频次】261