【摘要】 目的　研究用 4 0 0 0cGy高能电子线一次照射的大白鼠坐骨神经离体节段 (10 .0mm)原位再植后神经再生的可能性。方法　用 31只Wistar雌性大白鼠 ,1只大白鼠坐骨神经放射线照射后即行光镜、电镜观察 ;余 30只随机分成A组 (单纯手术组 )、B组 (放射手术组 )和C组 (正常对照组 ) ,A、B组经过术后 7、14、2 1、2 8、35、4 2、4 9、5 6、6 3和70天 ,C组饲养 1周 ,测定其坐骨神经功能恢复指标 (SFI)、电生理指标 ,进行组织学观察和计量分析。结果　 4 0 0 0cGy放射线严重损伤神经组织。A、B两组光镜下和大体所见无明显差异 ,均于术后 8～ 9周呈现神经再生 ,轴索形态良好 ,功能有一定程度恢复 ;两组计量资料比较无显著性差异 (P >0 .10 ) ,除SFI外各项指标均于术后一段时间达到C组均值水平。结论　经 4 0 0 0cGy高能电子线一次照射 10mm大白鼠坐骨神经离体节段原位再植不妨碍神经再生的速度和质量更多还原

【Abstract】 Objective To study the possibility of the regeneration of the sciatic nerves of the Wistar rats,which were replanted in situ after radiated by once 4 000cGy of high energy electronic line. Methods Thirty female Wistar rats were divided into 3 groups randomly, including A group(simple replantation ),B group (replantation after irradiation), and C group(without any treatment).The restored index of the sciatic nerve, electrophysiologic index were observed.The histological observation, measuring analysis were carried out.Results Large dose of radiation seriously injured the nerve tissue. In 8～9 weeks after the operation the rege nerating axon of A and B groups shaped well and the function of the whole nerve recovered in some degree, there was no significant difference between the two groups ( P >0.10), each index reached the average level of the C group sometime after the operation except SFI.Conclusion The replantation of the sciatic nerves of the Wistar rats in situ after radiated by a large dose of radiation once with high energy electronic line 4 000cGy does not hinder the rate and quality of the nerve regeneration.更多还原