A Novel Glucose Biosensor Based on Palladium Nanoparticles and Its Application in Detection of Glucose Level in Urine

【Abstract】 A novel glucose biosensor was constructed by electrodeposition of highly dispersed palladium （Pd） nanoparticles on a glassy carbon electrode （GCE）. Atomic force microscopy （AFM） was applied to characterize its surface morphology. Electrodeposited Pd nanoparticles exhibited efficiently electrocatalytic oxidation for hydrogen peroxide （H₂O₂） with relatively high sensitivity and stability,which was studied by CV technique and Raman spectroscopy,respectively. The GC/Pd/GOD/Nafion system allowed a low working potential of +0.3 V （vs. SCE）. Its signal current was linearly related to the glucose concentration in the range of 1.0×10 -6 —1.2×10 -4 mol·L -1 with a detection limit of 5.0×10 -7 mol·L -1 . The sensor required no special pretreatment to suppress interference from urate and L-ascorbate. It was successfully used in detection of glucose level in human urine with high stability,sensitivity and anti-poisoning ability .更多还原