【摘要】 目的　研究利用自制的合成抗原制备环孢霉素 A单克隆抗体的方法。方法　利用光化学反应将环孢霉素 A与聚 - L-赖氨酸偶联为合成抗原 ,免疫 BAL B/ c小鼠 ,将免疫后小鼠脾细胞与小鼠骨髓瘤细胞 SP2 / 0进行细胞融合 ,经反复筛选亚克隆获得抗 Cs A抗体的杂交瘤细胞株 ,以间接 EL ISA法进行抗体鉴定。结果　获得 4株分泌抗 Cs A抗体的杂交瘤细胞株 ,其中 2株分泌的抗体效价高 ,有一定特异性。结论　该抗原合成和免疫的方法切实可行 ,但单克隆抗体制备及保存方法有待进一步改进更多还原

【Abstract】 Objective To study the methods for preparing the monoclonal antibody against synthetic antigen of cyclosporine A. Methods These included using photochemical reaction to couple cyclosporine A with poly-L-lysine as synthetic antigen, immunizing BALB/c mice, fusing spleen cells and SP2/0 cells. Then hybridoma cells were repeatedly screened and subcloned. The monoclonal antibodies were identified by indirect ELISA. Results Four hybridoma cells in secreting anti-cyclosporine A monoclonal antibodies were obtained. Only two showed high titer and some specificity. Conclusions The methods of antigen synthesis and immunization were practicable. But the methods of antibody preparation and preservation need to be improved.更多还原