【摘要】 目的　探讨在体外培养液中不同细胞因子组合对脐血 CD34+ 细胞的扩增作用以及扩增后的 CD34+ 细胞的造血功能。方法　用 FL、SCF、TPO、IL - 3、TL - 6组合成不同的实验组 ,对脐血中分离纯化的 CD34+ 细胞经 6d的短期体外扩增 ,扩增后经流式细胞仪分析 ,L TC- IC、祖细胞集落群计数 ,从而求得较好的细胞因子组合组。结果CD34+ 细胞扩增倍数为 3 .1～ 8.6倍 ,16份脐血 CD34+ 细胞数达到 10× 10 6 以上的细胞总数 ;扩增的 CD34+ 细胞再造血功能与原始 CD34+ 细胞无显著差异。FL是扩增组合中不可缺少的细胞因子 ,同时还证实 TPO与其他细胞因子组合有扩增 CD34+ 细胞的作用 ,但单独使用 TPO稍长时间会使 CD34+ 细胞过多向巨核细胞分化。结论　 FST3 6细胞因子组合能使部份单位体积的脐血 CD34+细胞体外扩增达一定数量 ,为干细胞移植于成人提供了一定的实验依据与方法更多还原

【Abstract】 Objective To investigate the expansion of the CD 34 + cells of umbilical cord blood in vitro culture comprising different cytokines and the potential hematopoiesis function of the expanded CD 34 + cells. Method Expanding the purified CD 34 + cells from the fresh umbilical cord blood for 6 days in vitro with the use of different combination of FL, SCF, TPO, IL-3 and IL-6. Getting one better combination by analyses of the results from flowcytometry, LTC-IC, and progenitor clonogenic assays. Results The numbers of CD 34 + cells expanded by use of different combination of cytokines were 3.1-8.6 times as large as the numbers of ordinary ones,thus making 16 cases of insufficient CD 34 + cells become able to satisfy the needs of the adults weighing 50 kg. No significant difference was observed between the function of the expanded cells and the original ones in hematopoiesis. Meanwhile,it was proved that TPO in combination with other cytokines could strengthen the expansion of CD 34 + cells, but using TPO alone for long time culture would cause more CD 34 + cells to be megalocaryocytes. Conclusion The combination of FST36 cytokines can expand the CD 34 + cells of the umbilical cord blood to meet the adults’ needs in laboratory’s methods.更多还原