【摘要】 目的　探讨沙棘总黄酮改善高血压及慢性心力衰竭等疾病造成的心肌肥大的分子药理机制。方法　用机械牵张负荷离体培养大鼠心肌细胞 ,使心肌压力超负荷 ,造成肥大 ;并通过免疫组织化学方法观察不同浓度沙棘总黄酮对牵张诱导的心肌细胞 NF-κB激活的影响。结果　牵张离体培养心肌细胞 10 h后 ,NF-κB被激活 ;沙棘总黄酮抑制牵张诱导的心肌细胞 NF-κB的激活 ,其抑制作用与沙棘总黄酮存在浓度依赖关系。结论　沙棘总黄酮通过抑制 NF-κB信号传递系统的激活 ,引起细胞内相关分子表达调控机制改变 ,从而发挥其药理作用更多还原

【Abstract】 Objective To investigate the effect of total flavonoids of hippophae (TFH) on the improvement in myocardial hypertrophy. Methods Immunohistochemical method was used to assess the inhibitory effect of TFH on the activation of NF κB by stretching cultured cardiac myocytes. Results It was found that NF κB was activated by stretching cardiac myocytes in 10 hours; that TFH at the concentration of 1∶400 partly inhibited the activation of NF κB by stretching in cardiac myocytes; that TFH at the concentration of 1∶200 and 1∶100 completely inhibited the activation of NF κB by stretching in cardiac myocytes. Conclusion The results supported that the blockade of activation of NF κB might be a potential access to the improvement in myocardial function with the use of TFH for treatment of hypertension and chronic cardiac insufficiency.更多还原