【摘要】 目的　探讨部分背根切断术及针刺对背根节 (DRG) c- jun表达的影响。方法　将成年雄猫 15只 ,分假手术组 ,手术组和术后针刺组 ,每组 5只。手术组动物行单侧备用根术 ,术后针刺组动物经单侧备用根术后 ,针刺后肢手术侧 L6 脊神经分布区中的两组穴位 :足三里和悬中 ;伏兔和三阴交 ,每天一次 ,每次 30 min,连续针刺 7d。通过免疫组化 ABC法 ,用 c- jun特异抗体 (1∶ 10 0 0 ,Santa Cruz)对切片进行染色 ,DAB呈色。观察并测量 DRG中c- jun阳性神经元的分布、数量及反应强度。结果　在假手术组 DRG中 ,只有部分神经元染色。手术组 c- jun阳性神经元的数量较假手术组显著增多 (P<0 .0 5 ) ,其免疫反应水平较假手术组 ,显著上升 (P<0 .0 5 )。术后针刺组较手术组的 c- jun免疫反应强度增强 ,而阳性神经元的数量没有显著差异。结论　部分背根切断后备用 DRG神经元中c- jun的表达增加 ,针刺促使 c- jun的表达进一步上调更多还原

【Abstract】 Objective To investigate the change of c jun expression in spared dorsal root ganglion (DRG) following partial dorsal root rhizotomy and acupuncture. Methods Fifteen adult cats were divided into three groups with five cats in every group--sham operation, operation, and acupuncture following operation respectively. Animals of operation group were subjected to unilateral root rhizotomy(L 1 L 5,L 7 S 2 DRGs were sectioned, L 6 DRG was spared). Electro needling was performed alternatively at two sets of acupoints, including Zusanli and Xuanzhong, Futu and Sanyinjiao, located in the distribution area of spinal nerve L 6 on the operated side, 30 min everyday for 7 days following unilateral root rhizotomy. The animals of sham operation group experienced only skin and muscle incisions. On the 7th day after operation, all animals were sacrificed. The L 6 DRG on the disposal side of each animal was taken and made into frozen sections 20μm in thickness. The sections were stained under the same condition using specific c jun antibody (1:1000,Santa Cruz) by immunohistochemistry ABC method. The distribution and number as well as immunoreactive level of c jun positive neurons in DRG were observed and measured. Results In the L 6 DRG of sham operation group, only parts of neurons were stained. In contrast, the number of positive neurons and immunoreactive level for c jun increased significantly in operation group ( P <0 05). Compared with the operation group, the operation plus acupuncture group had higher c jun immunoreactive level of neurouns, but its number of c jun positive neurons didn’t change markedly. Conclusion Partial dorsal root rhizotomy leads to the up regulation of c jun expression in neurons of spared DRG, and acupuncture enhances the up regulation.更多还原