【摘要】 目的　获得中国成都地区汉族人群中短串联重复序列 ACTBP2 (SE33)位点的多态性分布 ,建立DHFRP2 、FIBRA和 ACTBP2 三个 STR位点的复合扩增方法。方法　用 Am p- FL P、聚丙烯酰胺凝胶电泳、银染技术 ,对 14 7个成都汉族无血缘关系个体进行分析。结果　发现 ACTBP2 位点的等位基因为 2 0个 ;基因型 86种 ;观察杂合度 (h) 0 .972 8;个体鉴别机率 (DP) 0 .986 1;多态性信息量 (PIC) 0 .9310 ;非父排除率 (EP) 0 .82 0 3。基因型分布皆符合 Hardy- Weinberg平衡 ,家系调查结果证明均符合孟德尔遗传法则。结论　 ACTBP2 位点多态性好 ,灵敏度高 ,可用于人类遗传分析及法科学中的亲子鉴定和个人识别 ,三个 STR位点的复合扩增方法简便 ,结果可靠更多还原

【Abstract】 Objective To obtain the polymorphism of ACTBP 2 locus in Han Chinese in Chengdu and establish the triplexing of three STR loci (DHFRP 2,FIBRA and ACTBP 2). Mothods Amp FLP, PAGE and silver stain were used to analyze 147 individuals. Results Twenty alleles and 86 genotypes were observed in ACTBP 2. The discriminating power(DP),observed heterozygosity(h),polymorphism information content(PIC) and chance of paternity exclusion power(EP) were 0.9861, 0.9728, 0.9310 and 0.8203, respectively. The distributions of the genotypes were in good agreement with Hardy Weinberg equilibrium. Studies of the families revealed that the locus was in accord with the Mendelian law. Conclusion These data indicate that ACTBP 2 is of good polymorphism and can be applied to human genetic study,forensic parentage testing and identification.更多还原