【摘要】 [目的 ]探讨河南省局部地区腹泻病人感染及携带大肠杆菌O15 7∶H7的情况 ,观察带菌时间及预后。[方法 ]采用流行病学监测方法发现病人 ,通过病人粪便mEC肉汤增菌 14h、胶体金免疫卡筛选、免疫磁珠法集菌、CHROMAGAR O15 7∶H7显色培养基分离、rfbO15 7、rfbO111、hlyA、stx1、stx2、eaeA引物PCR扩增方法进行毒力因子测定等方法 ,观察、研究感染病人的发病和预后。[结果 ]从 130 3份腹泻病人中共分离出的 38株O15 7∶H7菌株 ,检出率 2 9% ,PCRrfbO15 7扩增均为阳性。其中 2株具有stx2、hlyA和eaeA毒力基因 ,36株为O15 7∶H7不产毒株。[结论 ]我省首次从病人中分离出O15 7∶H7产毒株 ,病人发病后可于第 3～ 8d内检出病原体更多还原

【Abstract】 To explore the E coli O157∶H7 infection probability of diarrhea patients and to observe its carrying duration and prognosis in easten Henan \ The cases were found by epidemiological surveillance The samples were cultured in mEC broth for 14 h, screened with colloidal gold immunoassay test, amassed with immunomagnetic separation, isolated with CHROMAGAR O157∶ H7 culture medium, and rfbO157, rfbO111, hlyA, stx1, stx2 and eaeA primers were detected by PCR to observe and investigate the infection and prognosis \ 38 strains of O157∶H7 were isolated from 1 303 cases and rfbO157 showed positive result by PCR There exist stx2, hlyA and eaeA toxin gene in 2 strains from 38, and non producing toxin for the other 36 strains \ It is the first time to isolate O157∶H7 toxin producing strains in Henan Province and the strains can be detected on the 3rd to 8th day after illness更多还原