【摘要】 以环氧乙烷为羟乙基化试剂 ,在碱性水溶液中 ,对牛膝多糖 (Abps)进行羟乙基化 ,经过丙酮沉淀、膜分离、SephadexG 2 5柱层析等分离方法得到羟乙基化牛膝多糖 (Abps HE)纯品 ,检测其理化性质 ,并通过甲基化、GC MS分析 ,初步确证糖链中羟乙基主要取代在葡萄糖 6位和果糖的 1位或 6位上 .药理实验表明 ,羟乙基化牛膝多糖对荷Lewis肺癌小鼠NK活性具有一定促进作用更多还原

【Abstract】 Achyranthes bidentata polysaccharides (Achyran than, Abps) was hydroxyethylated by ethylene oxide in aqueous NaOH. The crude product was purified by acetone precipitation, dialysis, and Sephadex G-25 column chrom atography to give hydroxyethylated Abps (Abps-HE), which was identified by some physicochemical properties. By the methylation method and GC-MS analysis, it i s concluded that the hydroxyethyl groups are mainly substituted on 6-position o f the glucopyranose and fructofuranose of the sugar backbone. The product possesses a ntitumor activity and improves NK cell activity in Lewis lung cancer mice.更多还原