【摘要】 以H2 和N2 混合气体等离子体处理了聚丙烯微孔膜 ,采用扫描电镜观察了处理前后膜的形貌变化 ;真空全反射红外光谱和X射线光电子能谱证实在其表面接枝了大量活性氨基 ;用紫外光谱定量计算得知 ,所接枝的氨基浓度大约为 0 5μmol/cm2 .该改性膜可直接用于DNA的原位合成 ,其合成的DNA探针密度远高于功能化玻璃片基的探针密度 ,并可得到98%以上的偶联效率更多还原

【Abstract】 Polypropylene microporous membrane was treated with plasma in a mixture of nitrogen and hydrogen (1∶2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy (ATR FTIR), X ray photoelectron spectroscopy (XPS) and ultra violet (UV) spectra demonstrated the success of amino groups grafting. The density of active amino groups on the membrane surface is about 0 5 μmol/cm 2, which is much higher than that on modified glass slides. The as handled membranes were successively applied to the in situ oligonucleotides synthesis and an average coupling yield of more than 98% was achieved. These modified microporous membranes can be developed into a novel family of substrates for the in situ synthesis of DNA microarrays.更多还原