【摘要】 采用反相高效液相色谱技术 ,分离制备粗儿茶素粉末中表没食子儿茶素没食子酸酯( EGCG)、没食子儿茶素没食子酸酯 ( GCG)、表儿茶素没食子酸酯 ( ECG)三种酯型儿茶素馏分。制备色谱条件 :色谱柱 ,( 80 mm i.d× 5 0 0 mm,Fuji C18,粒径 2 5～ 40 μm) ;流动相 :有机醇 A- 1 /水( 1 6.5 /83.5 ,v/v,流速为 5 8m L/min;检测波长 2 78nm,进样量 30 0 0 mg。将所收集相应馏分分别经 P- 1大孔树脂吸附脱去溶剂 ,低沸点溶剂洗脱 ,旋转蒸发浓缩洗脱液 ,冷冻干燥后 ,得到 EGCG、GCG、ECG白色粉末。各物质经 HPLC检测纯度达 99.2 %以上。该法适用于分离纯化 EGCG、GCG、ECG三种酯型儿茶素单体更多还原

【Abstract】 Separation and preparation of (-) epigallocatechin(EGCG), (-) gallocatechin gallate(GCG) and (-) epicatechin gallate(ECG) from raw power of catechins were investigated by HPLC. Preparative chromatography conditions were: column(80 mm i.d×500 mm, Fuji C 18 , grain size 25~40 μm); mobile phase(alcohol A 1/water, 16.5/83.5, v/v ), flow rate(58 mL/min); detective wave length(278 nm); injection volume(10 mL). Under these conditions, three kinds of ester catechins were collected separately. Adsporptive resin P 1 was used to get rid of the solvent and low boiling point solvent was used to elute the above components. The eluted solution was evaporated and freeze dried under vacuum. Three kinds of ester catechin monomers can be obtained and their purity is more than 99.2%. The method is appicable for industrial production of ester catechin monomers-EGCG、GCG、ECG.更多还原