节点文献

两种新型镍-邻菲咯啉类核酸荧光探针的合成及性质研究

Synthesis and character of two new Ni-phenanthroline fluorescence probe for nucleic acid determination

推荐 CAJ下载
PDF下载
不支持迅雷等下载工具，请取消加速工具后下载。

【Author】 ZHUANGHui-sheng,GUO Chun-hua,CHEN Peng,WANG Qiong-e,CHEN Guo-nan(College of Environment Science and Engineering, Donghua University, Shanghai 250001; College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002; College of Chemistry and Chemical Engineering, Donghua University, Shanghai 250001), Huaxue Shiji, 2003, 25 (6), 325 -328

【机构】东华大学环境科学与工程学院；福州大学化学化工学院；东华大学化学化工学院；福州大学化学化工学院上海 200051福州大学化学化工学院；福建福州 350002；上海 200051；福建福州 350002；

【摘要】合成了咪唑并[f]邻菲咯啉(IP)和对羟基苯基咪唑并[f]邻菲咯啉(PHPIP)两种配体,并合成了两种新配合物Ni(Phen)2PHPIP和Ni(Phen)2IP,对其进行了表征,用紫外光谱、静态荧光光谱研究了配合物与小牛胸腺DNA的结合情况。两种配合物均与DNA有强烈作用,其中配合物Ni(Phen)2PHPIP在水溶液中几乎无荧光,而加入DNA后,能发射出很强的荧光,可以作为检测核酸分子的荧光探针。更多还原

【Abstract】 Two ligands , imidazo [f] 1, 10-phenanthroline (IP) and p-hydroxy- phenylimidazo[f] 1,10-phenanthro-line(PHPIP), and two new complexes , Ni(Phen)2 IP. 2ClO4,Ni(Phen)2 PHPIP.2ClO4 were synthesized and characterized . The binding of the complexes of the complexes to calf thymus DNA was investigated by using steady-state luminescence and ultraviolet visible absorption spectroscopy. The results indicated that the two complexes were bound to CT DNA . Ni(Phen)2 PHPIP showed almost no fluorescence in water , but it produced strong fluorescence when it was bound to CT DNA, so Ni(Phen)2 PHPIP is a prospective new kind of fluorescence probe for nucleic acid determination.更多还原

【关键词】荧光探针；小牛胸腺DNA； Ni(Phen)2PHPIP； Ni(Phen)2IP；
【Key words】 fluorescence probe； calf thymus DNA； Ni(Phen)2PHPIP； Ni(Phen)2IP；

【基金】国家自然科学基金资助项目(20277005);福建省自然科学基金资助项目(C0110005)

【文献出处】化学试剂 ,Chemical Reagents , 编辑部邮箱 ,2003年06期

【分类号】O627
【被引频次】29
【下载频次】285

知网节下载

节点文献中：

本文链接的文献网络图示:

本文的引文网络

节点文献