【摘要】 目的　探讨热疗对肿瘤细胞耐药性的产生和变化的影响。方法　采用RT_PCR法比较 4 1℃及 37℃下Tca8113及K5 6 2 ADM细胞株在体外接受短期化疗后 ,在mRNA水平上多药耐药基因 (MDR1)及多药耐药相关蛋白基因 (MRP)的表达情况 ,采用荧光分光光度计检测细胞内药物浓度的变化。结果　热疗化疗联合应用对Tca8113及K5 6 2 ADM细胞株抑制作用明显提高 ;4 1℃下热疗可在mRNA水平部分逆转K5 6 2 ADM细胞中MDR1和MRP的表达 ,并明显升高了K5 6 2 ADM细胞内药物浓度。结论　热疗不仅抑制肿瘤细胞耐药基因的表达 ,同时还有增加细胞内的药物浓度、拮抗耐药蛋白的作用 ,从而逆转耐药更多还原

【Abstract】 Objective\ The purpose of this study was investigate the effect of hyperthemia on multidrug resistance in K562/ADM cell.Methods\ The MDR1 (mulitdrug resistance gene) and MRP (multidrug resistant associated gene) gene expressions in Tca8113 and K562/ADM cell lines were analyzed by RT_PCR after treated with different cytotoxic drugs and different temperature (37 ℃ and 41 ℃). The function and expression of Pgp and MRP were detected by fluorescence photometeric assays.Results\ Inhibition rate of both cells was significantly enhanced by exposure to chemotherapeutic drugs and 41 ℃ temperature; Exposing to 41 ℃ hyperthemia reduced MDR1 and MRP expression and enhanced intracellular drug concentration as well in K562/ADM.Conclusion\ 41 ℃ hyperthemia could effectively enhance the inhibition rate of chemotherapeutic drugs and partially reverse the multidrug resistance. It is suggested that hyperthemia could be used as a method to overcome multidrug resistance.更多还原