【摘要】 目的　探讨静张应力环境对髁突软骨细胞增殖效应的影响。方法　将第 4代大鼠下颌髁突软骨细胞在细胞膜式张应力施加装置上培养 ,检测不同血清浓度及持续不同的静张应力 (5kPa、10kPa)在 0～ 12h内对髁突软骨细胞增殖活性的影响。结果　低血清培养基中 ,随培养时间的延长 ,硅胶膜上髁突软骨细胞的增殖活性受到抑制 ;短期 (2h)的静张应力 (5kPa、10kPa)对髁突软骨细胞细胞周期的调控影响不大 ;0～ 10h内 5kPa静张应力组软骨细胞增殖指数随着张应力作用时间的延长不断上升 ,增殖指数峰值位于 10h处 ;0～ 8h内 10kPa静张应力组软骨细胞增殖指数随着张应力作用时间的延长不断上升 ,增殖指数峰值位于 8h处 ;5kPa静张应力组较 10kPa静张应力组对髁突软骨细胞具有更大的促增殖作用。结论　静张应力可以调节髁突软骨细胞的增殖活性更多还原

【Abstract】 Objective\ The purpose of this study was to investigate the effects of static tension_stress on proliferation of mandibular condylar chondrocytes in rats.Methods\ The fourth_passage chondrocytes were harvested from the mandibular condyles of 2_week_old SD rats. A continuous static tension_stress was applied on the cells in vitro using a cellular static tension_stress device, and the proliferation of mandibular condylar chondrocytes were examined using a flow cytometry. The other 30 specimens in the control group were divided into six groups to examine the effects of calf serum on cellular static tension_stress without any stress. The experimental group consisted of 70 specimens which were divided into seven groups under continuous static tension_stress (5kPa, 10kPa) for 0 to 12 hours. Multivariable analyses were conducted to test the associations between proliferation of condylar chondrocytes and different continuous static tension stresses.Results\ The results showed that the lower calf serum inhibited the proliferation of rat mandibular condylar chondrocytes. There was little effect on the proliferation of chondrocytes under continuous static tension_stress (5kPa, 10kPa) for 2 hours. The proliferation of mandibular condylar chondrocytes was promoted as the application time of stress was prolonged (0～10 hours in 5kPa groups and 0～8 hours in 10kPa groups). The maximal proliferation appeared, when the condylar chondrocytes were cultured under 5kPa continuous static tension_stress for 10 hours, and 8 hours under 10kPa stress (P<0.05). The proliferation of cells in the 5kPa group was more obvious than in the 10kPa group (P<0.05).Conclusion\ The data prove that mechanical stimulates in vitro can influence and regulate the growth of condylar chondrocytes. It provides experimental evidence for advanced study on cellular mechanical research in functional orthopedics.\;更多还原