【摘要】 目的　研究人白细胞介素 1受体拮抗剂 （hIL₁ra）基因转染人胚颞颌关节软骨细胞的方法和表达。方法　分离培养人胚颞颌关节软骨细胞 ,将hIL₁ra基因以阳离子脂质体Lipefectine介导进行基因转染 ,免疫细胞化学染色和ELISA法检测转染细胞中hIL₁ra蛋白的表达。结果　使用阳离子脂质体介导hIL₁ra基因转染人胚颞颌关节软骨细胞 ,免疫细胞化学染色hIL₁ra蛋白呈阳性表达 ,瞬时转染和稳定转染时 ,转染细胞胞浆内和培养基上清液中均有目的基因的表达 ,与未经转染的对照组存在显著性差异 （P <0 0 1）。结论　体外实验证实脂质体介导hIL₁ra基因转染方法切实可行 ,为今后颞颌关节骨关节病的基因治疗提供了依据。更多还原

【Abstract】 Objective\ The purpose of this study was to investigate methods and expression of the recombinant human interleukin₁ receptor antagonist gene transfected into chondrocytes of temporomandibular joints （TMJ）.Methods\ Chondrocytes derived from the TMJ of 5～7 months old human fetus were transfected by recombinant human interleukin₁ receptor antagonist gene via cationic liposome （Lipefectine） as a medium. The expressing level of hIL₁ra protein was detected using immunocytochemistry and enzyme_linked immunosorbent assay （ELISA）.Results\ The positive results of the immunocytochemistry were found. The positive expression was detected in the cell plasma and the cell culture supernatant, and there was significant difference between the cells with and without gene transfection （P<0.01）.Conclusion\ This feasible method provides experimented evidence for the future gene therapy of temporomandibular joint osteoarthrosis.\;更多还原