【摘要】 目的观察大鼠脑出血灶周围组织BaxmRNA(Bax基因 )、Bcl 2mRNA(Bcl 2基因 )表达的变化规律及参麦注射液的干预影响。方法立体定位于大鼠苍白球内注射Ⅶ型胶原酶 ,诱导大鼠脑出血。采用半定量逆转录酶聚合链反应 (RT PCR) ,检测脑出血后 1 2h、1d、2d、4d、7d参麦组与各对照组血肿周围组织的BaxmRNA、Bcl 2mRNA表达的影响。结果脑出血后 1 2～2 4h,模型组BaxmRNA表达显著上调 ,1 2h达到高峰 ,2d后逐渐减弱 ,7d时仍高于假手术组水平 ;模型组Bcl 2mRNA 1 2h时明显升高 ,1d时达到高峰 ,2d时降至假手术组水平 ;参麦组脑出血后 1～ 2d时BaxmRNA表达低于模型组 ,但 1 2h～ 4d 4个时间点Bcl 2mRNA表达均高于模型组 (P <0 .0 5 )。结论脑出血后 ,Bax、Bcl 2基因表达均有上调 ,与脑出血灶周围组织损伤有一定关系 ,参麦注射液可以上调Bcl 2mRNA的表达 ,下调BaxmRNA的表达更多还原

【Abstract】 ?Objective To investigate the intervening effects of Shenmai Injection(SI) on the expression of Bax and Bcl 2 genes in the tissue around the experimental intracerebral hemorrhage(ICH) among rats. Methods ICH models were prepared by injecting Type Ⅶ of collagnase into the golbas palliclus of right side among rats and the animals were then divided into two groups, with SI used to those in treating group. Then, the expressive levels of Bax and Bcl 2 genes in the tissue around ICH were determined at 12?h, 1?d, 2?d, 4?d and 7?d respectively following the treatment with RT PCR method and a comparison of the results was made between the two groups. Results The expressive level of Bax mRNA was significantly elevated in the animals of model group, with a peak value seen at 12?h and a reducing tendency after this point till 7?d, when it remained at a higher expressive level than normal. On the other hand, the level of Bcl 2 mRNA was also significantly elevated in this group, with the peak value seen at 1d and a reducing limitation to normal level at 2?d. In contrast, the expressive level of Bax mRNA in the animals of SI treating group was lower than that of model group till the duration 1d to 2d, while that of Bcl 2 mRNA in treating group was always higher than that of animals in model group till 4d(P<0.05). Conclusions The expressive levels of Bax and Bcl 2 genes are all upregulated following ICH and hold some degrees of association with damage to the tissue around the ICH focus. For this case, SI is able to upregulate the expression of Bcl 2 mRNA and to downregulate that of Bax mRNA . 〔更多还原