【摘要】 目的:研究雷公藤内酯醇对体外培养的牛晶状体上皮细胞增殖的抑制作用。方法:取第4代对数生长期的牛晶状体上皮细胞培养24h后,加入重组人表皮生长因子(终浓度50μg/L)和不同浓度的雷公藤内酯醇(40,20,10μg/L),分别作用6,12,24,48,72h后,采用MTT法观察雷公藤内酯醇对牛晶状体上皮细胞增殖的影响。结果:不同浓度的雷公藤内酯醇,于不同时间作用在处于增殖状态的LECs,其增殖抑制率升高呈现明显的时间及剂量依赖性。结论:雷公藤内酯醇对晶状体上皮细胞增殖有明显的抑制作用在防止后囊混浊方面有广阔的前景。更多还原

【Abstract】 Objevtive To study the inhibitory effects of Triptolide on bovine lens epithelial cell BLECs proliferation in vitro. Methods: The fourth generation of BLECs during logarithmic period were incubated for 24 hours, added to recombinant Human Epithelial Growth Factor rhEGF and followed by exposed to different concentrations of Triptolide (40?20?10 μg/L),for 6,12,24,48,72 hours respectively. The inhibitory effects of Triptolide on BLECs were determined by MTT colorimetric assay. Results The inhibitory rates of BLECs proliferation were increased in both time-dependent and dose-dependent ways by Triptolide in vitro. Conclusion Triptolide could effectively inhibit the proliferation of BLECs in vitro and it might have broad prospects in the prevention of posterior capsual opacification.更多还原