【摘要】 目的 :研究NO合成酶抑制剂L NAME对大鼠睡 /醒周期的调控以及GABA在其中的作用。方法 :用多导睡眠描记和皮下给药的方法 ,观察NO合成酶抑制剂L NAME ,GABA受体激动剂muscimol和baclofen对大鼠睡 /醒周期的影响 ,以及预先应用GABA受体激动剂后 ,对L NAME的睡眠抑制效应的调节。结果 :L NAME能抑制大鼠的SWS和REMS ,REMS出现次数也减少 ,muscimol或baclofen对大鼠的SWS无明显影响 ,但均使REMS时间缩短 ,出现次数减少。预先应用muscimol或baclofen后 ,可使L NAME单独作用时对睡眠的抑制作用消失。结论 :GABA受体激动剂似能拮抗NO合成酶抑制剂对大鼠睡眠的影响。更多还原

【Abstract】 Objective: To testify the effect of N G nitro L arginine methyl ester (L NAME) (an inhibitor of nitric oxide synthase) and GABA on the regulation of the sleep/waking cycle in adult rats implanted for chronic sleep recordings.Methods: L NAME, muscilmol(an agonist of the receptor of GABA A) and baclofen (an agonist of the receptor of GABA B) were injected subcutaneously, and their effects on sleep/waking cycle were observed. The effects of pretreatment with muscimol or baclofen on the changes of sleep and waking induced by L NAME were also observed. Results: Subcutaneous administration of L NAME reduced slow wave sleep (SWS), rapide eye movement sleep (REMS) and the number of REMS periods.Subcutaneous adminstration of muscimol or baclofen did not induce significant changes of SWS, but both of them reduced REMS and the nulmber of REMS periods. Pretreatment with muscimol or baclofen eliminated the reduction of REMS induced by subcutaneous administration of L NAME.Conclusion: It seems that the agonists of GABA receptors can antagonize the suppression of sleep induced by L NAME.更多还原