【摘要】 目的 :观察罗格列酮对高脂喂养胰岛素抵抗 (IR)大鼠动物模型的胰岛素增敏作用及对解偶联蛋白(UCPs)基因表达的影响。方法 :2 4只大鼠随机分为普通饮食组 ,高脂对照组 ,罗格列酮组。后 2组给予高脂饲料喂养 ,制作胰岛素抵抗 (IR)模型。 4周后取罗格列酮组大鼠给予罗格列酮 (8mg·kg-1·d-1)灌胃 ,其他 2组给予安慰剂灌胃 2周。以高胰岛素 正葡萄糖钳夹实验评价胰岛素敏感性的变化 ,用半定量的RT PCR检测UCPs基因mRNA的改变。结果 :罗格列酮组与高脂对照组比 ,血清游离脂肪酸降低 (P <0 .0 5 ) ,葡萄糖输注率增加 (P <0 .0 1)。与高脂对照组比 ,罗格列酮组大鼠肌组织UCP 3基因mRNA表达增高 4 6 .2 % ,肝脏UCP 2基因表达增加 34.9% ,且体重增加明显。结论 :罗格列酮有降低游离脂肪酸、改善胰岛素敏感性的作用 ,但可引起体重增加。胰岛素增敏作用可能与UCPs基因表达增强有关更多还原

【Abstract】 Aim:To explore the effect of rosiglitazone, a ligand of peroxisome proliferator activated receptors γ (PPARγ) , on improving insulin sensitivity and uncoupling proteins (UCPs) gene mRNA expression in high fat rat model of insulin resistance.Methods:The rats were allocated into three groups,eight rats in each group. One group was given standard food and the other 2 groups were given high fat diet for making insulin resistance model. One of the groups given high fat diet were treated once by gavage with rosiglitazone(8 mg·kg -1 ·d -1 ) for 14 days after four week high fat diet. The change of insulin sensitivity was valued by hyperinsulinemic euglycemic clamp. The change of UCPs mRNA in liver and muscle was measured using RT PCR. Results:Compared with high fat group, the level of FFA in rosiglitazone group was lower( P <0.05), and the glucose infusion rate was higher ( P <0.01).UCP 3 mRNA in rosiglitazone group increased by 46.2 % in muscle and UCP 2 mRNA increased by 34.9% in liver and body weight for rats in rosiglitazone group was increased. Conclusion:Rosiglitazone can reduce the level of serum FFA, improve insulin sensitivity,but increase the body weight. The effect of the improved sensitivity may involve in the overexpression of UCPs gene.更多还原