【摘要】 目的对深圳市HIV-I流行毒株亚型监测分析。方法应用巢式PCR方法对26份1998年10月-1999年8月采集的深圳市HIV感染者的外周静脉血淋巴细胞样品的基因进行扩增,获得了HIV-1膜蛋白基因(env)的核进片段,并对其C2-V3区及邻近350-450个核苷酸序列进行了测定和分析。结果26份样品中,7份为B亚型、5份为C亚型、14份为E亚型。结论深圳地区人群中流行的HIV-1毒株归属于E、B、C三种亚型,尚未发现其它亚型。更多还原

【Abstract】 Peripheral blood mononuclear cells from26HIV-positive individuals in Shenzhen area were extracted.nested polymerase chain reaction(PCR)technique was performed to amplify a proviral DNA fragment of the HIV-1envelope gene encoding the C2-V3region and neighboring sequence.Result:In comparison with the consensus sequences of international subgroups,the twenty-six strains were classified into three subtypes,namely subtype E(n=14),subtype B(n=7)subtype C(n=5).更多还原