【摘要】 目的 :研究匹罗卡品致痫后大鼠行为、EEG及大脑组织学变化特征。方法 :SD大鼠匹罗卡品腹腔注射 ,诱发急性癫痫持续状态 (SE)后 ,观察慢性期自发性发作 ;描记EEG ;Neo Timm染色观察海马苔藓出芽 ,Nissl染色观察海马神经元损伤。结果 :注射匹罗卡品后 ,87%的动物呈现SE ,持续 6～ 2 4h后这一部分大鼠均出现慢性自发发作 ,组织学检查发现海马有显著的神经元损伤 ,齿状回内分子层苔藓纤维出芽。结论 :匹罗卡品模型基本复制了人类颞叶癫痫的临床病理特征 ;SE所致的海马结构性损伤及苔藓纤维重构是自发性发作形成的基础更多还原

【Abstract】 Objective To characterize the acute and chronic behavioral, electrographic and histological changes of sustained seizures induced by pilocarpine in rats. Methods The rats in the study were divided into the experimental group and control group. After status epilepticus (SE) was induced in the experimental rats, the surviving animals were continuously monitored for 6 h～60 days. At different times after the pilocarpine injection, the animals were processed for neo Timm and Nissl staining to visualize granule cell mossy fiber sprouting and hippocampal cell damage. Results Of the animals injected with pilocarpine, 87% developed SE, and most of the pilocarpine induced SE rats (20% 100%) showed recurrent seizures during the chronic period. A widespread cell loss was noted in the hippocampal formation of the rats with pilocarpine induced SE.The pattern of neo Timm staining in the inner molecular layer was clearly altered in animals that showed pilocarpine induced SE. The Neo Timm staining score of the experimental group was significantly higher than that of the control group. The degree of inner molecular layer mossy fiber terminal staining increased with time after the epileptogenic lesion. Conclusion The temporal lobe epilepsy model induced by pilocarpine in rats can replicate several of the features of human temporal epilepsy (hippocampal cell loss, inner molecular layer mossy fiber sprouting, and spontaneous recurrent seizures), and it may be a useful model for studying this human temporal lobe epilepsy. The results also suggest that structural brain damage insulted by pilocarpine induced SE may underlie or be associated with recurrent spontaneous seizures in rats.更多还原