【摘要】 制备了雌二醇抗体 (抗E2 6 CMO BSA)和12 5I放射配体E2 6 CMO histamine 12 5I,建立了血清雌二醇放射免疫分析方法 ,其特点是通过微量标记法提高标记物比活度 ,不用乙醚萃取直接测定血清样本 ,简化了测量步骤 ,缩短了测量时间 ,且试剂稳定时间可延长至 2个月以上。应用本法可以快速测量动物及人的血清样本 ,2 5～ 3h即可得出结果。更多还原

【Abstract】 A sensitive radioimmunoassay for estradiol was set up without extraction The antisera were rabbits anti estradiol 6 CMO BSA with a cross reaction ratio of 0 6% against estriol Pre synthesized estradiol 6 CMO His was labeled with 125 I in a tiny volume (17 5μl) Highly purified radioactive (3550μCi/μg) estradiol 6 CMO His 125 I was obtained This estradiol radioimmunoassay kit is stable for at least two months It can be used for studying animal endocrinology and clinical endocrinology更多还原