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⁶⁰Coγ辐射对菜心离体培养的影响

EFFECT OF ⁶⁰ Co γ IRRADIATION WITH SEED AND SHOOT-TIP OF Brassica campestris L.var ON ITS CULTURE IN VITRO

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【Author】 LIAO Fei\|xiong\+1\ YU Rang\|cai\+2\ PAN Rui\|chi\+3 (1.Floriculture Research Institute, Guangdong Academy of Agricultural Science, Guangzhou, Guandong prov. 510640; 2.Irradiation Center in South China Agricultural University, Guangzhou Guangdong prov. 510640; 3. College of Life Science, South China Normal University, Guangzhou, Guangdong prov. 510631)

【机构】广东省农业科学院花卉研究所；华南农业大学辐照中心；华南师范大学生命科学院广东广州510640；广东广州510640；广东广州； 510631；

【摘要】菜心种子经⁶⁰Coγ射线辐照在离体培养中茎尖存活率随剂量增大而下降 ,茎尖增殖、子叶愈伤组织诱导和愈伤组织分化受到抑制 ;外植体龄影响离体培养中的辐射效应 ;小于 50Gy直接辐照离体培养中的茎尖促进芽的增殖。从对离体培养的效应看 ,对种子的适宜辐射剂量是200Gy左右 ,经催芽种子为 50～100Gy,离体培养茎尖可用 40～ 70Gy。更多还原

【Abstract】 The survival rate in vitro of shoot tips of Brassica campestris L.var from seeds irradiated by 60 Co γ rays decreased with the increase of dose. Irradiation inhibited proliferation of shoot tip, induction of callus from cotyledons and differentiation of the callus. The age of explant contributed to the effect of irradiation in the culture. Irradiation stimulated the proliferation of shoot tip with dose less than 50Gy. Based on the effect of irradiation in the tissue culture, the effective dose recommended was about 200Gy for seeds , 50～100Gy for pre soaked germinating seeds and 40～70Gy for shoot tips in vitro , respectively.更多还原