【摘要】 以兰考泡桐组织培养苗叶片为材料,分别采用CTAB-Ⅰ、CTAB-Ⅱ、SDS-Ⅰ、SDS-Ⅱ四种方法提取叶片DNA,并对不同提取结果进行紫外分光光度、电泳、酶切、RAPD等方法的鉴定。结果表明,用四种方法提取的泡桐叶片DNA得率约为3.5-5.2μg/10mg,A260/A280约为1.7-1.8,A260/A230在2.0以上,分子量在23Kb以上,能够被EcoRⅠ、HindⅢ酶切,能够进行RAPD分析。综合考虑得率、纯度、质量等因素,以SDS-Ⅰ法为最佳方案。更多还原

【Abstract】 Using the leaves from plantlet of Paulownia in vitro as the material,four different methods were compared in extraction of Paulownia genomic DNA, which belonged to two kinds,CTAB and SDS. The prepared DNA samples were tested by ultraviolet spectrophotometer analysis, agarose gel electropHoresis, restriction \{enzyme\} digestion and RAPD reaction. The results showed that as for the four samples, the production rate were \{3.5\}-5.2μg per ten milli-gram of fresh leaves,A260/A280 =1.7-1.8,A260/A230>2.0,the molecular weights above 23 Kb, and they were all suitable for digesting with restrictive enzyme and RAPD analysis. However,\{integrative\} factors considered,the best one should be SDS-Ⅰcorrespondingly.更多还原