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彗星试验对4种化学物质诱导人淋巴细胞DNA损伤的检测

Detection for DNA Damage in Human Lymphocytes Induced by Four Chemicals Using Comet Assay

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【作者】 杨东平张美辨何继亮

【Author】 YANG Dong-ping ZHANG Mei-bian,HE Ji-liang,et al.Medical School of Jia Xing College,Jiaxing,Zhejiang314001,China

【机构】 嘉兴学院医学院浙江大学医学院嘉兴学院医学院 浙江嘉兴314001浙江杭州310031浙江嘉兴314001浙江杭州310031

【摘要】 目的验证彗星试验可否用于检测不同类型化学诱变剂诱发的DNA损伤。方法人淋巴细胞经4-硝基喹啉氧化物(4-NQO,拟紫外线诱变剂)、甲基甲烷磺酸酯(MMS,烷化剂)、博来霉素(BLM,拟X射线诱变剂)和丝裂霉素C(MMC,DNA交联损伤剂)染毒3h,再培养0h或21h,然后用彗星试验检测这4种化合物所诱发的DNA单链断裂(SSB)。结果彗星试验能立即检出4-NQO、MMS和BLM诱发的SSB,并具有剂量-效应关系(P<0.01),在21hDNA损伤程度减轻(与0h比较,P<0.01)。MMC诱发的DNA损伤在染毒后21h才被检出,并有剂量-效应关系(P<0.01)。结论虽然这4种化学诱变剂的作用机制不同,但彗星试验仍能检测出他们诱发人淋巴细胞DNA损伤的效应。

【Abstract】 Objective To validate feasibility of comet assay as a tool for detecting DNA damage induced by various types of chemical mutagens.Study of DNA damage induced by4chemicals on human lymphocytes was carried out in vitro.Methods Human lymphocytes were exposed to4-nitroquinoline-1-oxide(4NQO,a UV-mimetic agent ),methyl methanesulfonate(MMS,an alkylating agent ),Bleomycin(BLM,a radiamimetic agent )and Mitomycin(MMC,a DNA crosslink agent )for3h,the DNA single strand breaks(SSB)induced by4chemicals were measured immediately(0h-incubation)and21h-incubation after3h-exposure to the chemicals with comet assay.Results It was found that the SSB induced by4NQO,MMS and BLM,which revealed a dose-response relationship(P<0.01),could be detected immediately after the3h-exposure with comet assay,and the SSB diminished at the end of21h-incubation after the exposure(comparing with0h-incubation,P<0.01).The SSB induced by MMC could not be detected until21h after exposure with comet assay,and also showed a dose-response relationship(P<0.01).Con clusion Although the mutagenic mechanisms of4chemical mutagens were different,their DNA damage effects on human lymphocytes could be detected with comet assay.

【基金】 科技部中意国际合作项目(国科外字[2000]0120;No1);浙江省科技厅国际合作项目(012104)
  • 【文献出处】 环境与健康杂志 ,Journal of Environment and Health , 编辑部邮箱 ,2003年01期
  • 【分类号】R394.6
  • 【被引频次】8
  • 【下载频次】209
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