【摘要】 利用Iodogen法成功地对N-琥珀酰亚胺 3-(三正丁基锡)苯甲酸酯(ATE)进行了放射性碘-125标记,利用Sep-Pak硅胶柱实现了干扰蛋白标记的ATE及Iodogen与S125IB的分离,得到了放射性碘间标蛋白质有用的中间体S125IB,标记率93%以上。并研究了影响标记的因素,得出较佳标记条件为:ATE与 Na125I摩尔比6:1、氧化剂 Iodogen用量7mg、室温反应5min。更多还原

【Abstract】 N-Succinimidyl 3-(tri-n-butylstannyl)benzoate (ATE) was radioiodinated using Iodogen as oxidant and useful conjugate S125IB of labeling proteins was obtained . ATE and Iodogen affecting labeling proteins were successfully isolated from S125IB by Sep-Pak silica . The labeling efficiency was more than 93%. Several factors affecting labeling such as labeling time, the amount of Iodogen and the mole ratio of ATE to Na125I, were studied. The better labeling conditions were obtained as follows: mole ratio of ATE to Na125I = 6:1, Iodogen=7mg , labeling time = 5min in room temperature.更多还原