节点文献
银杏叶苯丙氨酸解氨酶盐析条件的研究
Study on the Condition for Salting-out of Phenylalanine Ammonia-lyase(PAL) in Ginkgo biloba Leaves
【摘要】 用固体加入法研究了银杏叶苯丙氨酸解氨酶（PAL）的盐析过程。结果表明: 50％→75％饱和度的（NH4）2SO4盐析,蛋白质的收获率高,PAL的纯化倍数高,是较理想的盐析范围。
【Abstract】 The course of salting-out the phenylalanine ammonia-lyase(PAL) in Ginkgobiloba leaves was studied. The results showed that there were more protein product and higher purifying fold of PAL at the range of 50%→75% (NH4)2SO4 saturation which was the suitable range for PAL salting-out.
【关键词】 银杏叶；
苯丙氨酸解氨酶；
盐析条件；
【Key words】 Ginkgu biloba leaf； phenylalanine ammonia-lyase(PAL)； salting-out；
【Key words】 Ginkgu biloba leaf； phenylalanine ammonia-lyase(PAL)； salting-out；
【基金】 教育部科技攻关计划资助项目(02095);湖北省自然科学基金(2002AB094)
- 【文献出处】 湖北农学院学报 ,Journal of Hubei Agricultural College , 编辑部邮箱 ,2003年06期
- 【分类号】S664.3
- 【被引频次】10
- 【下载频次】156