【摘要】 采用代表性差异分析方法 ,以小菜蛾的敏感品系作为驱赶扩增子 ,抗杀螟丹近等基因系作为检测扩增子 ,通过 3轮消减杂交得到大小为 15 0～ 30 0bp的差异片段 ,经亚克隆测序并与GenBank等数据库同源比较 ,发现其中一个亚克隆序列与P4 5 0基因有部分同源性 ,其余亚克隆与已知基因序列的同源性较低 ,判断这些序列可能是新基因的片段。更多还原

【Abstract】 The genomic difference between Plutella xylost el la L. susceptible strain and cartap resistance near isogenic line strain w as preliminarily analyzed by representational difference analysis (RDA) technique. In RDA system, the driver amplicon is susceptible strain while the tester amplicon is cartap resistance ne ar isogenic line strain. After three rounds of subtractive hybridization, the di fferent products were obtained and their sizes were about 150-300 bp. Compariso ns between our sub clone sequencing results and GenBank show that only one sub clone sequence has P450 gene homology, other sub clone sequences show no homo logy to knew gene. Preliminary judgment revealed that these segments might be ne w gene sequences.更多还原