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黄瓜染色体制片及倍性研究
Study on Chromosome Preparing and Ploidy in Cucumber
【摘要】 以黄瓜卷须为材料,对黄瓜染色体制片中的一些主要参数和方法进行了研究,建立了黄瓜染色体制片的系统操作规程。研究发现,黄瓜染色中心直径和异染色质数目与植株倍性呈正相关,可作为黄瓜倍性鉴定的间接指标,其中单倍体染色中心直径平均为5 1μm,双单倍体为13 3μm,单倍体异染色质数目平均5 0,双单倍体为11 9。
【Abstract】 Main parameters and methods of chromosome preparing with wall degradation hypotonic technique in cucumber were studied,and a suitable method was established.The results indicated that there was a evident correlationship between chromocenter diameter,heterochromatin number and plant ploidy level.The chromocenter diameter and heterochromatin number of haploid and double haploid are 5.1,5.0 μm and 13.3,11.9 μm respectively.
【基金】 天津市重点攻关项目(993122511-3)
- 【文献出处】 华北农学报 ,Acta Agriculturae Boreall—sinica , 编辑部邮箱 ,2003年01期
- 【分类号】S642.2
- 【被引频次】81
- 【下载频次】574