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转甜菜碱醛脱氢酶(BADH)基因小麦的耐盐耐旱性
Salt and Drought Stress Tolerance in Transgenic Wheat Expressing Betaine Aldehyde Dehydrogenase Gene
【摘要】 采用室内模拟盐、旱胁迫,结合田间实际测定的方法,对基因枪法获得的转BADH基因小麦多个株系的不同世代材料进行了耐盐、耐旱性鉴定。结果表明,在旱、盐胁迫条件下,转BADH基因小麦在种子萌发、幼苗生长、根系发育以及质膜保护等方面均比受体品种(对照)具有明显的优势;在田间生长条件下的转基因株系,其叶片蒸腾强度比对照明显降低,而离体叶片失水速率与对照没有明显差别。
【Abstract】 Assay for salt tolerance and drought resistance of transgenic wheat expressing betaine aldehyde dehydrogenase,obtained through particle gun method,were conducted under simulated salt/drought stress condition.The results indicated that the transgenic wheat has many obvious advantages over its donor plants,such as the greater germinating ability under osmotic stress up to -0.3 Mpa,the more vigorous development of seedlings,especially the well developed roots under drought stress condition,as well as the improved plasma membrane protection of excised leaf and the lower transpiration rate under field condition.But the water loss rate for excised leaf was not obviously different from its donor control plants.
【Key words】 Wheat; Transgenic; Transgenic wheat; BADH; Drought Resistance; Salt Tolerance;
- 【文献出处】 华北农学报 ,Acta Agriculturae Boreall—sinica , 编辑部邮箱 ,2003年01期
- 【分类号】S512.1
- 【被引频次】73
- 【下载频次】351