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转甜菜碱醛脱氢酶(BADH)基因小麦的耐盐耐旱性

Salt and Drought Stress Tolerance in Transgenic Wheat Expressing Betaine Aldehyde Dehydrogenase Gene

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【作者】 张艳敏郭北海蒋春志温之雨丁占生李辉李洪杰何锶洁陈受宜朱至清

【Author】 ZHANG Yanmin1,GUO Beihai1,JIANG Chunzhi1,WEN Zhiyu1,DING Zhansheng1,LI Hui1,LI Hongjie1,HE Sijie2,CHEN Shouyi2,ZHU Zhiqing3(1Institute of Food and Oil Crops Research,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031;2Insitute of Genetic and Developmental Biology Sciences,Chinese Academy of Science,Beijing 100101;3.Institute of Botany,Chinese Academy of Science,Beijing 100091)

【机构】 河北省农林科学院粮油作物研究所中国科学院遗传发育研究所中国科学院植物所 河北石家庄050031河北石家庄050031北京100101北京100091

【摘要】 采用室内模拟盐、旱胁迫,结合田间实际测定的方法,对基因枪法获得的转BADH基因小麦多个株系的不同世代材料进行了耐盐、耐旱性鉴定。结果表明,在旱、盐胁迫条件下,转BADH基因小麦在种子萌发、幼苗生长、根系发育以及质膜保护等方面均比受体品种(对照)具有明显的优势;在田间生长条件下的转基因株系,其叶片蒸腾强度比对照明显降低,而离体叶片失水速率与对照没有明显差别。

【Abstract】 Assay for salt tolerance and drought resistance of transgenic wheat expressing betaine aldehyde dehydrogenase,obtained through particle gun method,were conducted under simulated salt/drought stress condition.The results indicated that the transgenic wheat has many obvious advantages over its donor plants,such as the greater germinating ability under osmotic stress up to -0.3 Mpa,the more vigorous development of seedlings,especially the well developed roots under drought stress condition,as well as the improved plasma membrane protection of excised leaf and the lower transpiration rate under field condition.But the water loss rate for excised leaf was not obviously different from its donor control plants.

【关键词】 小麦转基因BADH耐盐性耐旱性
【Key words】 WheatTransgenicTransgenic wheatBADHDrought ResistanceSalt Tolerance
【基金】 国家863计划(2001AA212121-3);植物转基因及其产业化专项资助课题(J99-B-010)
  • 【文献出处】 华北农学报 ,Acta Agriculturae Boreall—sinica , 编辑部邮箱 ,2003年01期
  • 【分类号】S512.1
  • 【被引频次】73
  • 【下载频次】351
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