【摘要】 以5个基因型不同的小麦幼胚、幼穗作为供试材料,探讨了品种基因型、ABA处理及生根条件等对小麦离体培养的影响。结果表明,小麦品种豫麦18 64为遗传转化的良好受体材料;0 2mg/LNAA和0 8mg/LIAA可促进幼苗生根;在幼胚愈伤组织诱导前14d,用0 5mg/LABA处理可有效抑制出芽率,并有利于促进胚愈伤组织形成和再生能力的保持。更多还原

【Abstract】 The acceptors were obtained from 5 wheat cultivars including Yumai 1864,Yumai 34,Wenmai No.6,Yumai 70 and 895004.The factors affecting the plants regeneration,such as genotype,ABA treatment and rooting conditions were studied.The result showed that Yumai 1864 was the best acceptor genotype among 5 cultivars.The hormone proportions of NAA 0.2 mg/L and IAA 0.8 mg/L could efficiently improve root differentiation.Meanwhile it was found in the experiments that immature embryo germination was not only inhibited,but embryonic callus development could also be significantly improved by culturing callus on the medium containing ABA(0.5 mg/L)for two weeks.更多还原