【摘要】 通过RT PCR,获得了小麦黄花叶病毒罗田分离物28kD蛋白基因的cDNA克隆pUW28 10。序列分析结果表明,小麦黄花叶病毒不同分离物的28kD蛋白基因的核苷酸序列存在一定的差异:湖北罗田和河南潢川分离物在第326,327和336位较四川雅安、江苏扬州及日本分离物缺少3个核苷酸(nt),前两者核苷酸长度为762nt,后三者为765nt;不同分离物核苷酸序列同源性从92 1%到96 9%,相应的氨基酸序列同源性从89 8%到97 3%。更多还原

【Abstract】 A cDNA clone (pUW2810) containing 28 kD protein gene from Luotian isolate of wheat yellow mosaic virus(WYMV)was obtained by RTPCR.Sequence analysis revealed that nucleotides sequence of 28 kD protein gene varied among different isolates of wheat yellow mosaic virus.28 kD protein genes of the Luotian and Huangchuan isolates comprised 762 nucleotides(nt)and were less 3 nt than others,located at the site of 326,327 and 336 nt,respectively.The different isolates shared homologies ranging from 92.1% to 96.9% in nucleotide sequence and 89.8% to 97.3% in amino acid (aa) sequence.更多还原