【摘要】 探讨痴呆模型鼠海马的突触素改变及其与学习记忆的关系。切断成年SD大鼠左侧穹窿海马伞(Fimbria-Fomix,FF),建立隔海马胆碱能系统损害的痴呆模型。损伤4周后,用Y型迷宫检测大鼠学习记忆能力,免疫组织化学染色和图象分析技术等方法对大鼠海马突触素进行定量分析。结果表明,损伤组学习记忆能力(3.82±0.64)明显低于正常组(8.81±0.32)(P<0.01);与正常组相比,损伤组损伤侧海马CA1区多形层、辐射层、腔隙分子层和齿状回分子层突触素免疫反应物的光密度明显降低,其光密度值分别是43.40±2.57;40.2l±3.22;12.37±1.87;26.63±2.36(P<0.01),并与其学习记忆能力呈显著正相关。本研究结果提示,痴呆模型鼠海马突触素与学习记忆的关系密切。更多还原

【Abstract】 Objective: To study change of synaptophysin ( SYH ) in hippocampus of rats with fimbria-fornix ( FF) transection and its impacts on learning and memory. Methods; adult SD rats were subjected to left FF transection. After four weeks, the learning and memory abilities were measured by Y-maze test and the brains of rat were processed for synaptophysin immunocytochemistry. In this study, we used computer-assisted optical density measurements of synaptophysin immunostaining. Results: the learning and memory abilities decreased significantly in comparison with control group (P < 0. 01) , the value of COD ( correct optical density) of SYH immunolabel of the oriens, radiatun, lacunosum and the molecular lay of dentate gyrus ( MDG) in lesion group decreased significantly in comparison with control group ( P < 0. 01) , and the value of COD of SYH immunolabel in hippocampus of lesion group is positively correlated with the learning and memory deficits. Conclusion: the decrease of synaptophysin in hippocampus of rats with FF transection may be responsible for its learning and memory deficits.更多还原